作者：罗琴¹, 贾广成², 杨金漆¹, 高海莹¹, 陈鹏飞¹, 陈祥贵¹, 何绍志³

作者单位：1. 西华大学食品与生物工程学院，四川 成都 610039;
2. 新疆维吾尔自治区计量测试研究院，新疆 乌鲁木齐 830011;
3. 四川省食品检验研究院，四川 成都 611731

关键词：茶多酚;磁性固相萃取;离子液体;表面修饰

摘要：

茶多酚是茶叶中最重要且含量最高的生物活性成分，探索新的茶多酚分离方法有利于提高其开发利用率，并推动产业技术革新。基于磁性固相萃取技术和离子液体在分离领域的诸多优势，该研究以咪唑类脯氨酸离子液体作为功能成分对磁性介孔二氧化硅纳米颗粒进行表面修饰制备一种新型的磁性吸附剂，通过扫描电子显微镜，傅里叶变换红外光谱仪，X-射线衍射仪和振动样品磁强计对其结构进行表征。静态吸附实验结果表明该吸附剂对茶多酚的最大吸附量为213.99 mg/g；吸附动力学和热力学研究结果表明该吸附过程更符合准二级动力学方程和Freundlich吸附等温模型。研究结果可以为建立新型的茶多酚分离方法提供一定的参考。

Preparation of ionic liquid-functionalized magnetic nanoparticles for tea polyphenols adsorption
LUO Qin¹, JIA Guangcheng², YANG Jinqi¹, GAO Haiying¹, CHEN Pengfei¹, CHEN Xianggui¹, HE Shaozhi³
1. School of Food and Bioengineering, Xihua University, Chengdu 610039, China;
2. Xinjiang Uygur Autonomous Research Institute of Measurement and Testing, Urumqi 830011, China;
3. Sichuan Institute of Food Inspection, Chengdu 611731, China
Abstract: Tea polyphenols (TP) is the most important bioactive constituent in tea, so the investigation of new separation methods could promote the production of the tea polyphenols significantly and have a meaningful value. Based on the merits of magnetic solid phase extraction and ionic liquid in the field of separation, in the present study, a novel magnetic adsorbent was successfully prepared using magnetic mesoporous silica nanoparticles as magnetic core and N-methyl imidazole proline salt as the surface functional components, and its structural features were characterized by Scanning Electron Microscope, Fourier Transformed Infrared spectrum, X-ray Diffractometer, and Vibrating Sample Magnetometer. According to static adsorption experiments, it was found that the most excellent adsorption capacity for TP reached 213.99 mg/g under the optimal conditions. The adsorption equilibrium data fitted the pseudo-second-order kinetic and Freundlich models well. These results can provide a reference for establishing a new separation method of TP.
Keywords: tea polyphenols;magnetic solid phase extraction;ionic liquid;surface-modification
2022, 48(9):71-77  收稿日期: 2021-11-09;收到修改稿日期: 2022-01-16
基金项目: 西华大学大健康管理促进中心开放课题（DJKG2019-007）
作者简介: 罗琴（1998-），女，四川广元市人，硕士研究生，专业方向为天然产物的开发与利用
参考文献
[1] YANG C S, WANG X, LU G, et al. Cancer prevention by tea: animal, studies, molecular mechanisms and, human relevance[J]. Nature Reviews Cancer, 2009, 9(6): 429-39
[2] KHAN N, MUKHTAR H. Tea polyphenols for health promotion[J]. Life Sciences, 2007, 81(7): 519-533
[3] 凌益春, 孙达, 王林善. 茶多酚生产工艺比较分析[J]. 中国茶叶, 2010, 32(5): 12-14
[4] EARLE M J, SEDDON K R. Ionic liquids. Green solvents for the future[J]. Pure & Applied Chemistry, 2009, 72(7): 10-25
[5] 张霞, 黄新异, 郝泉, 等. 离子液体在高速逆流色谱分离制备天然产物中的研究进展[J]. 天然产物研究与开发, 2020, 32(9): 1621-1627
[6] 万常, 李启慧, 曾超珍, 等. 基于离子液体与超声波辅助提取普洱茶茶多酚的工艺优化[J]. 茶叶通讯, 2021, 48(3): 494-500
[7] ZHANG W, FENG X T, ALULA Y, et al. Bionic multi-tentacled ionic liquid-modified silica gel for adsorption and separation of polyphenols from green tea (Camellia sinensis) leaves[J]. Food Chemistry, 2017: 637-648
[8] 陈冰, 肖维毅, 尧珍玉, 等. 磁性多壁碳纳米管固相萃取-气相色谱质谱联用快速测定食用油中的苯并[a]芘[J]. 中国测试, 2015, 41(11): 44-49
[9] CHEN P F, YAO S, ZHENG D M, et al. Fabrication of a novel core-shell-shell temperature-sensitive magnetic composite with excellent performance for papain adsorption[J]. RSC Advances, 2021, 11: 24843-24851
[10] LIU J B, YAO S, WANG L T, et al. Adsorption of bromophenol blue from aqueous samples by novel supported ionic liquids[J]. Journal of Chemical Technology and Biotechnology, 2014, 89(2): 230-238
[11] FARIMANI M H R, SHAHTAHMASEBI N, ROKNABADI M R, et al. Study of structural and magnetic properties of superparamagnetic Fe₃O₄/SiO₂ core-shell nanocomposites synthesized with hydrophilic citrate-modified Fe₃O₄ seeds via a sol-gel approach[J]. Physica E, 2013, 53: 207-216
[12] Kuo C Y, Wu C H, Wu J Y. Adsorption of direct dyes from aqueous solutions by carbon nanotubes: Determination of equilibrium, kinetics and thermodynamics parameters[J]. Journal of Colloid and Interface Science, 2008, 327: 308-315
[13] 朱兴一, 刘晓平, 谢捷, 等. 竹叶纤维吸附法制备茶多酚的研究[J]. 浙江工业大学学报, 2013, 41(6): 605-609+613
[14] HU X C, DU X F. Adsorption of Tea Polyphenols using Microporous Starch: A Study of Kinetics, Equilibrium and Thermodynamics[J]. Molecules, 2019, 24(8): 1449-1461