作者：许晓燕, 余梦瑶, 罗霞

作者单位：四川省中医药科学院菌类药材系统研究与开发实验室 中药品质评价与创新中药研究四川省重点实验室，四川 成都 610041

关键词：灵芝;指纹图谱;抗肿瘤;谱效关系

摘要：

基于偏最小二乘回归分析法（PLS）分析灵芝子实体体外抗肿瘤活性谱效关系。采用HPLC法建立灵芝药材指纹图谱，评价不同灵芝子实体对人肺腺癌细胞A549的细胞增殖抑制率，运用PLS法关联指纹图谱共有峰的相对峰面积与A549细胞增殖抑制IC₅₀，建立谱效关系模型。结果表明：灵芝子实体指纹图谱中有14个共有峰，其中有6个共有峰的回归系数与A549细胞抑制IC₅₀呈负相关，其贡献大小排序为X₅（灵芝烯酸B）＞X₈（灵芝酸A）＞X₁₂（灵芝酸D）＞X₄＞X₉（灵芝酸H）＞X₂（灵芝酸C₂）。灵芝子实体体外抗肿瘤药效作用是多种成分共同作用的结果，可为灵芝药材质量控制提供参考。

Analysis of fingerprint and antitumor activity in vitor of Ganoderma lucidum by HPLC
XU Xiaoyan, YU Mengyao, LUO Xia
Laboratory for Systematic Research and Development of Fungus Medicine, Sichuan Academy of Chinese Medicine Sciences Sichuan Provincial Key Laboratory of Quality Evaluation and New Drug Creation of Traditional Chinese Medicine, Chengdu 610041, China
Abstract: To explore the relationship between HPLC fingerprint and antitumor activity in vitro of the alcohol extraction of Ganoderma lucidum fruit bodies by PLS. The fingerprint of Ganoderma lucidum was established by HPLC. The inhibitory rate of A549 of Ganoderma lucidum was evaluated. PLS analysis was used to study the relationship between the HPLC fingerprint and the antitumor activity.  A total of 14 common substance peaks were identified, and 6 of them were negatively correlated with the Growth inhibition IC₅₀ values of A549 cells. The contribution order is X₅ (Gano¹¹derenic acid B) > X₈ (Ganoderic acid A) > X₁₂ (Ganoderic acid D) > X₄ > X₉ (Ganoderic acid H) > X₂ (Ganoderic acid C₂). The antitumor activity of Ganoderma lucidum is the result of many components. This study can provide reference for quality control of the medicinal materials.
Keywords: Ganoderma lucidum;fingerprint;antitumor activity;spectrum-effect relationship
2020, 46(10):23-27,36  收稿日期: 2020-05-12;收到修改稿日期: 2020-06-24
基金项目: 国家现代农业产业技术体系四川食用菌创新团队项目(SCCXTD-2020-07)；省科技基础条件平台项目(2019JDPT0012)；省公益性科研院所基本科研业务专项(A-2018N-23)；省公益性科研院所基本科研业务专项(A-2019N-23)
作者简介: 许晓燕(1980-),女,重庆市人,副研究员,硕士,主要从事中药标准和质量评价研究
参考文献
[1] 蔡晓翠, 毛艳, 贺金华, 等. 新疆一枝蒿不同极性部位体外抗炎作用的谱-效关系分析[J]. 药物分析杂志, 2019, 39(9): 1580-1589
[2] 李戎, 闫志勇, 李文军, 等. 创建中药谱效关系学[J]. 中医教育, 2002, 21(2): 62
[3] 黄潇, 刘婧, 吴建华, 等. 基于灰色关联法分析栀子提取物抗炎作用谱效关系[J]. 中成药, 2020, 42(1): 233-237
[4] 杨秋霞,杨运云,刘耀慧, 等. 傅立叶变换离子回旋共振质谱法对赤灵芝的化学成分鉴定和指纹图谱研究[J]. 分析测试学报, 2019, 38(5): 525-531
[5] ZHAO Y, YOU X M, JIANG H, et al. Spectrum–effect relationships between high-performance liquid chromatography fingerprints and anti-inflammatory activities of Leontopodium leontopodioides (Willd.) Beauv.[J]. Journal of Chromatography B, 2019, 1104: 11-17
[6] 伍利华, 钟林江, 杨俊莉, 等. 基于体外抑菌作用的金莲花系列制剂谱-效关系研究[J]. 中国测试, 2019, 45(11): 51-56
[7] 刘肖雁, 姜文文, 蒋海强, 等. 基于“谱-效-代”关联的参枝苓口服液质量标志物的初步研究[J]. 中草药, 2019, 50(19): 4603-4612
[8] 肖瑞瑶, 滕攀攀, 胡超, 等. 偏最小二乘回归分析应用于中药谱效关系的研究进展[J]. 辽宁化工, 2020, 49(3): 303-305
[9] 林志彬. 灵芝抗肿瘤作用的免疫学机制及其临床应用[J]. 中国药理学与毒理学杂志, 2015, 29(6): 865-882
[10] 李淑娟, 刘宏壁, 李晓云, 等. 灵芝醇提取物的抗肿瘤活性研究[J]. 华西药学杂志, 2018, 33(2): 175-178
[11] 余梦瑶, 许晓燕, 魏巍, 等. 38株灵芝子实体抗肿瘤质量的生物评价[J]. 中国食用菌, 2015, 34(2): 47-51
[12] 徐宾, 贾薇, 王忠, 等. 灵芝酸A对前列腺癌LNCaP细胞的生长抑制作用及机制[J]. 菌物学报, 2019, 38(5): 717-727
[13] JIANG J, GRIEB B, THYAGARAJAN A. Ganoderic acids suppress growth and invasive behavior of breast cancer cells by modulating AP-1 and NF-κB signaling[J]. International Journal of Molecular Medicine, 2008, 21(5): 577-584
[14] ZHENDONG L, LIANG L, BEI X. Effect of ganoderic acid D on colon cancer Warburg effect: Role of SIRT3/cyclophilin D[J]. Elsevier B. V, 2018, 824: 72-77
[15] DAO L L, YING J L, DONG H Y. Ganoderma lucidum derived ganoderenic acid B reverses ABCB1-mediated multidrug resistance in HepG2/ADM cells[J]. International Journal of Oncology, 2015, 46(5): 2029-2038