作者：廖彬1, 杨佳怡2, 陈桂芳2, 高运华2, 王晶2, 任歌2
作者单位：1. 北京化工大学，北京 100029;
2. 中国计量科学研究院，北京 100029
关键词：染色质可及性;测序;ATAC-seq;数据分析
摘要：
在真核生物中，染色质作为遗传物质DNA的载体，直接参与基因的表达调控。染色质的可及性是指染色质中的DNA能与转录因子等蛋白复合物结合的区域，对染色质可及性的测定可为获取开放区域的信息、核小体定位信息以及转录因子结合信息提供基础依据。随着高通量测序技术的进步以及测序成本的降低，研究者已开发多种研究染色质可及性的测序方法，该文介绍4种常见的染色质可及性测序方法，对其中的染色质转座酶可及性测序(assay for transposase-accessible chromatin with high-throughput sequencing，ATAC-seq)的原理和数据分析方法进行重点描述，讨论ATAC-seq的应用与发展趋势，为染色质可及性以及基因表达调控的研究提供参考。
Research progress and data analysis of assay for transposase-accessible chromatin withhigh-throughput sequencing
LIAO Bin1, YANG Jiayi2, CHEN Guifang2, GAO Yunhua2, WANG Jing2, REN Ge2
1. Beijing University of Chemical Technology, Beijing 100029, China;
2. National Institute of Metrology, China, Beijing 100029, China
Abstract: Chromatin, as the carrier of genetic material DNA, is directly involved in the regulation of gene expression in eukaryotes. The accessibility of chromatin is the region where DNA in chromatin can bind to protein complexes such as transcription factors. The determination of chromatin accessibility provides a basic basis for obtaining open region information, nucleosome positioning information and transcription factor binding information. With the progress of high-throughput sequencing technology and the reduction of sequencing cost, researchers have developed a variety of sequencing methods to study chromatin accessibility. This paper presents four common methods of chromatin accessibility sequencing, focuses on the principle and data analysis methods of ATAC-seq, discussing the application and development trend of ATAC-seq. It provides a reference for the study of chromatin accessibility and gene expression regulation.
Keywords: chromatin accessibility;sequencing;ATAC-seq;data analysis
2020, 46(10):4-10 收稿日期: 2020-07-20;收到修改稿日期: 2020-08-29
基金项目: 国家自然科学基金（31900433，41907272）
作者简介: 廖彬(1998-),男,广西南宁市人,硕士研究生,专业方向为计算化学、生物学
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