作者：段凤敏1, 张绍旺1, 邵维炯2, 高华磊2, 匡兴贵2, 饶杰1, 保志娟2
作者单位：1. 云南省计量测试技术研究院, 云南 昆明 650228;
2. 云南农业大学烟草学院, 云南 昆明 650201
关键词：大叶种;晒青毛茶;茶多酚;响应面分析;抗氧化活性
摘要：
以云南凤庆大叶种晒青毛茶为原料，在单因素实验的基础上，通过Box-Behnken实验设计及响应面分析法，研究乙醇浓度、液料比、提取温度、提取时间对晒青毛茶茶多酚提取率的影响，同时通过超氧阴离子、羟基自由基和DPPH自由基的清除能力评价晒青毛茶茶多酚的抗氧化性能。结果表明，0.2 g茶粉在固定超声波功率为160 W下，预测最佳提取工艺为：乙醇浓度60.01%，液料比49.28∶1 mL/g，超声时间25 min，超声温度30℃，总多酚得率为19.905%（验证值为19.83%）。云南大叶种晒青毛茶茶多酚具有很强的抗氧化能力，对超氧阴离子、羟基自由基、DPPH自由基清除的IC50值分别为7.96 μg/mL、7.22 μg/mL、0.433 μg/mL。
Optimization of extraction technology and antioxidant activity study of tea polyphenols in sun-dried green tea from Fengqing large-leaved species in Yunnan Province
DUAN Fengmin1, ZHANG Shaowang1, SHAO Weijiong2, GAO Hualei2, KUANG Xinggui2, RAO Jie1, BAO Zhijuan2
1. Yunnan Institute of Measuring and Testing Technology, Kunming 650228, China;
2. College of Tobacco Science, Yunnan Agricultural University, Kunming 650201, China
Abstract: Using sun-dried green tea from Fengqing large-leaved species in Yunnan Province as raw material, a Box-Behnken design and response surface analysis was applied to study the effects of ethanol concentration, liquid material ratio, extractive temperature and extractive time on the extraction rate of tea polyphenols based on the single factor experiments. And the antioxidant activity of tea polyphenols were evaluated using the scavenging activity superoxide anion (O2-), ·OH and DPPH· free radical ability. The results showed that when the ultrasonic power was set at 160 W with 0.2 g tea powder, the predictable optimum ultrasonic extraction conditions for tea polyphenols were as follows:ethanol concentration 60.01%, liquid/solid ratio of 49.28:1 mL/g, ultrasonic time 25 min, and ultrasonic temperature 30℃. Under this optimum condition, the total extraction yield was 19.905% (the tested value is 19.83%). The tea polyphenols of sun-dried green tea from Fengqing large-leaved species had an obvious antioxidant activity, the IC50 value of superoxide anion, ·OH and DPPH free radical scavenging ability was 7.96 μg/mL, 7.22 μg/mL and 0.433 μg/mL, respectively.
Keywords: large leaf species;sun-dried green tea;tea polyphenols;response surface analysis;antioxidant activity
2019, 45(5):66-73 收稿日期: 2018-12-14;收到修改稿日期: 2019-01-25
基金项目: 国家质检总局科技计划项目（2017QK191）；云南农业大学大学生科技创新创业训练计划项目（2018-180）
作者简介: 段凤敏(1978-),女,云南凤庆县人,高级工程师,硕士,从事化学计量及标准物质研究
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