作者：高运华¹, 黎朋¹, 侯东军², 张玲¹, 王晶¹

作者单位：1. 中国计量科学研究院, 北京 100013;
2. 中国动物疫病预防控制中心, 北京 100125

关键词：配方奶粉; 核苷酸; 高效液相色谱; 定量

摘要：

研究建立了定量测定配方奶粉中5种核苷一磷酸(钠盐)的高效液相色谱方法。通过添加适量乙酸、离心浓缩、调节pH等技术方法,快捷有效地去除奶粉中影响核苷酸含量定量测量的蛋白质、脂肪及其他杂质成分,使用核苷酸国家级标准物质,采用外标法对样品中的核苷酸含量进行了定量测量。结果表明:该方法检出限低,准确度在90.8％～99.3％之间,方法的重复性限为1.43,再现性限为2.44。该方法准确度高,重复性好,可行性强,易于掌握,并且快捷、成本低,为奶粉中核苷酸含量的定量测量及相关产品的质量技术监督检验提供了方法学依据。

Quantitative determination of nucleotides in formula milk powder by HPLC method

GAO Yun-hua¹, LI Peng¹, HOU Dong-jun², ZHANG Ling¹, WANG Jing¹

1. National Institute of Metrology, Beijing 100013, China;
2. China Animal Disease Control Center, Beijing 100125, China

Abstract: A quantitative method for determining adenosine 5'-monophosphate, cytidine 5'-monophosphate, guanosine 5'-monophosphate, uridine 5'-monophosphate and inosine 5'-monophosphate in formula milk powder was described. Following deproteinisation, adjusting pH, and so on, the sample extracted was quantitatively analyzed by the reversed-phase liquid chromatography. The CRM of nucleotides was used as the standard for quantitative analysis. Performance parameters included recoveries of 90.8%～99.3%, and repeatability limit as 1.43, and reproducibility limit as 2.44. The described method has been applied to the analysis of infant formula milk powder.

Keywords: formula milk powder; nucleotides; HPLC; quantitative

2012, 38(1): 44-47  收稿日期: 2011-5-18;收到修改稿日期: 2011-7-27

基金项目: 国家质检总局食品安全专项基金(ASPAQ1101)科技部科技支撑计划项目(AZC0710-2)

作者简介: 高运华(1973-),男,山东定陶县人,副研究员,主要从事生物测量相关标准物质研制和法制计量工作。

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