作者:向省维1, 巫明毫2, 张力3, 刘海鲨4, 陈雪珂4, 刘玲1, 郭义东5, 林家富5, 宋涛5, 郑德志4, 沙菁洲4
作者单位:1. 成都大学食品与生物工程学院,四川 成都 610052;
2. 凉山彝族自治州第一人民医院药剂科,四川 西昌 615000;
3. 四川省生态环境科学研究院,四川 成都 610046;
4. 四川省固体废物与化学品管理中心,四川 成都 610032;
5. 成都大学药学院,四川 成都 610052
关键词:沱江;抗生素残留;微生物群落;多样性
摘要:
为调查评估沱江流域水体中抗生素残留水平和生态风险,并探究抗生素对微生物群落结构和多样性的影响。以沱江流域6个国级/省级水环境质量监测点为采样点,分析水体抗生素浓度,评估环境风险,并研究残留抗生素与微生物群落结构之间的相关性。结果显示,沱江流域水体中抗生素检出浓度在0.71 ~177.98 ng/L,这可能与城市生活废水与养殖企业废水的排放有关,中下游区域抗生素的检出浓度远高于上游区域。6个监测点位中有4个处于高风险等级。高通量测序结果显示,Shannon指数、Simpson指数和Chao1等微生物多样性指数在中游最高,下游最低;变形菌门、拟杆菌门和放线菌门是水体中优势细菌种群,在SHM监测点中种群丰度占比高达92.72%。水体中部分抗生素种类与水体微生物种群分布相关性显著,微生物群落结构受残留抗生素影响。
Effects of antibiotic residues on microbial diversity and ecological risk assessment in Tuojiang river
XIANG Shengwei1, WU Minghao2, ZHANG Li3, LIU Haisha4, CHEN Xueke4, LIU Ling1, GUO Yidong5, LIN Jiafu5, SONG Tao5, ZHENG Dezhi4, SHA Jingzhou4
1. School of Food and Biological Engineering, Chengdu University, Chengdu 610052, China;
2. The First People’s Hospital of Liangshan Prefecture, Xichang 615000, China;
3. Sichuan Provincial Department of Ecology and Environment, Chengdu 610046, China;
4. Sichuan Solid Waste and Chemicals Management Center, Chengdu 610032, China;
5. School of Pharmacy, Chengdu University, Chengdu 610052, China
Abstract: In order to evaluate the concentration and ecological risk of residual antibiotic in Tuojiang river, and analyze the relevance between the residual antibiotic and microbial diversity, six national or provincial water environmental quality monitoring sites were chosen. As results showed, the concentration of residual antibiotic was 0.71 -177.98 ng/L, which was mainly related to discharge of domestic or aquaculture wastewater. The concentration of residual antibiotics in upper reaches of Tuojiang river is lower than other sites. Meanwhile, four in six sampling sites showed high ecological risk. The results of High-throughput sequencing showed, the microbial diversity indexes such as Shannon index, Simpson index and Chao1 index, were the highest in the middle reaches and the lowest in the lower reaches, Proteobacteria、Bacteroidetes、Actinobacteria was the dominant population. The population abundance in SHM site was higher to 97.72%. In conclusion, there is a significant correlation between some antibiotics and the distribution and diversity of microbial in surface water, and the structure of microbial communities are affected by residual antibiotics.
Keywords: Tuojiang river;antibiotic residual;microbial community;diversity
2023, 49(2):72-79 收稿日期: 2022-07-25;收到修改稿日期: 2022-09-22
基金项目: 四川省环境抗生素残留量对环境微生态的影响研究及风险预测项目(2019HB16)
作者简介: 向省维(1997-),女,云南昭通市人,硕士,主要从事抗生素残留相关研究工作
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