作者：李姝1,3, 王松涛1,2, 张娜3, 贾俊杰1, 焦威4, 刘怡3, 赵建3, 沈才洪1,2
作者单位：1. 泸州品创科技有限公司，四川 泸州 646000;
2. 国家固态酿造工程技术研究中心，四川 泸州 646000;
3. 四川大学生命科学学院 资源微生物学与生物技术教育部重点实验室，四川 成都 610064;
4. 中国科学院成都生物研究所，四川 成都 610041
关键词：ADS-7大孔树脂;使用寿命;苦荞;黄酮
摘要：
研究ADS-7大孔树脂连续使用对苦荞精制品中黄酮成分的影响。以黄酮吸附量、吸附率及苦荞精制品中芦丁、烟花苷、槲皮素和山奈酚4种黄酮成分含量等为指标，结合AlCl3分光光度法及高效液相色谱法，考察ADS-7大孔树脂富集苦荞黄酮的使用次数及对主要黄酮成分含量的影响。检测结果显示，ADS-7大孔树脂在连续使用30次后，黄酮吸附量仅降低8.9 %，黄酮吸附率降低9.3 %，树脂吸附性能较好，且苦荞精制品中芦丁、烟花苷、槲皮素和山奈酚4种黄酮成分的总含量在树脂连续使用25次内变化波动不大。检测结果表明，采用ADS-7型大孔树脂纯化苦荞黄酮，无需经再生处理，可连续使用至第25次。该方法环保、经济，可为苦荞黄酮精制品的规模化生产提供参考。
The effect of continuous use of ADS-7 macroporous resin on flavonoids in refined tartary buckwheat products
LI Shu1,3, WANG Songtao1,2, ZHANG Na3, JIA Junjie1, JIAO Wei4, LIU Yi3, ZHAO Jian3, SHEN Caihong1,2
1. Luzhou Pinchuang Technology Co., Ltd., Luzhou 646000, China;
2. National Engineering Research Center of Solid-State Brewing, Luzhou 646000, China;
3. Key Laboratory of Biological Resource and Ecological Environment of Chinese Education Ministry, College of Life Science, Sichuan University, Chengdu 610064, China;
4. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
Abstract: To study the effect of continuous use of ADS-7 macroporous resin on flavonoids in refined tartary buckwheat products. Combining AlCl3-spectrophotometry with high performance liquid chromatography, the flavonoid adsorption capacity, adsorption rate, and the content of four flavonoids (rutin, kaempferol-3-O-rutinoside, quercetin and kaempferol) in refined tartary buckwheat product were used as indicators to investigate the life of ADS-7 macroporous resin in purifying tartary buckwheat flavonoids and its effect on the content of main flavonoids. The results showed that after 30 times repeated use of ADS-7 macroporous resin, the flavonoid adsorption capacity was only reduced by 8.9%, and the flavonoid adsorption rate was reduced by 9.3%, indicating that the resin has better adsorption performance. In addition, the total content of four flavonoids in refined tartary buckwheat products did not change much within 25 consecutive uses of the resin. In conclusion, the ADS-7 macroporous resin can be used to purify tartary buckwheat flavonoids continuously until the 25th time without regeneration treatment. This method is environmentally friendly and economical, and provides a reference for the industrial production of tartary buckwheat flavonoids.
Keywords: ADS-7 macroporous resin;service life;tartary buckwheat;flavonoids
2022, 48(6):58-63 收稿日期: 2021-03-29;收到修改稿日期: 2021-05-27
基金项目: 泸州老窖股份有限公司资助项目（2017k-95）；泸州品创科技有限公司资助项目（18H1314）
作者简介: 李姝（1991-），女，湖北宜昌市人，工程师，博士研究生在读，研究方向为天然产物与药理
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