作者：谭佳淇, 何小慧, 赵江林, 李丽娇, 涂文应, 鲍志杰, 李强

作者单位：成都大学食品与生物工程学院 农业农村部杂粮加工重点实验室，四川 成都 610106

关键词：高通量测序;藜麦;内生菌;多样性;群落结构

摘要：

为揭示藜麦成熟期和幼苗期各部位内生菌的群落结构及分化情况，采用高通量测序技术对藜麦不同生长时期组织样本的内生菌进行16S rRNA基因V3-V4区测序。结果表明，藜麦成熟期的根部细菌Observed species指数、Shannon指数和Chao1指数均显著高于藜麦幼苗期（P<0.05）；成熟期和幼苗期的藜麦根部细菌Observed species指数、Shannon指数、Simpson指数和Chao1指数显著高于同时期的藜麦茎、叶细菌（P<0.05）；根部内生菌多样性和群落结构与茎部、叶部、籽粒存在较大差异。其中，蓝细菌门（Cyanobacteria）呈现叶部>茎部>根部的趋势，而变形菌门（Proteobactrtia）则呈现相反的趋势。研究结果较为全面地解析了藜麦成熟期和幼苗期各部位内生菌的群落结构和分化情况，可对藜麦的栽培提供参考。

Analysis of endophytic bacterial community structure in different growth stages of quinoa
TAN Jiaqi, HE Xiaohui, ZHAO Jianglin, LI Lijiao, TU Wenying, BAO Zhijie, LI Qiang
Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, School of Food and Biological Engineering, Chengdu University, Chengdu 610106, China
Abstract: In order to reveal the community structure and differentiation of endophytes in different parts of quinoa at the mature and seedling stages, the endophytes of quinoa were sequenced by using high-throughput sequencing technology. The results showed that the bacterial diversity indexes of quinoa at mature stage were significantly higher than that of quinoa at seedling stage, including observed species, Shannon index and Chao1 indexes (P<0.05). The bacterial diversity indexes of quinoa root were significantly higher than that of quinoa stem and leaf at the mature and seedling stages, including observed species, Shannon index, Simpson index and Chao1 index (P<0.05). The diversity and community structure of root endophytes were different from that of stems, leaves and grains. The abundance of Cyanobacteria was the highest in leaves, followed by stems and roots. While the Proteobactertrtia had the highest abundance in quinoa roots, followed by stems and leaves. The results comprehensively analyzed the community structure and differentiation of endophytic bacteria in different parts of quinoa at the mature and seedling stages, which provided a reference for the cultivation of quinoa.
Keywords: high-throughput sequencing;quinoa;endophytes;diversity;community structure
2022, 48(1):73-79  收稿日期: 2020-10-09;收到修改稿日期: 2020-12-08
基金项目:
作者简介: 谭佳淇（2000-），女，四川广安市人，专业方向为食品科学与工程
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