作者:胡晓月, 屈泽华, 黄红香
作者单位:复旦大学高分子科学系 聚合物分子工程国家重点实验室,上海 200438
关键词:积分球;荧光量子产率;测试条件优化
摘要:
采用QM40型荧光光谱仪,以罗丹明6G和硫酸奎宁作为标准样品,从仪器参数设置(步长、积分时间和狭缝)以及激发波长等几个方面,探讨采用积分球测量荧光量子产率时的最优测试条件选择。测试发现:荧光量子产率的准确率与步长和积分时间的改变并无线性关系,选择较长的步长和较短的积分时间可以缩短测试时长;荧光强度的改变对具有高荧光量子产率的物质影响更为明显,因此测试中应控制其荧光强度不宜过高;而激发波长的改变对荧光量子产率的数值影响较大,建议在测试时应固定激发波长的位置。该研究可为获得可靠的荧光量子产率数据提供一定的参考。
Study of the optimized test conditions for fluorescence quantum yield measuring by integrating sphere
HU Xiaoyue, QU Zehua, HUANG Hongxiang
State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200438, China
Abstract: By using QM40 as fluorescence spectrometer, and Rhodamine 6G and quinine sulfate as standard samples, the optimized test conditions of the measurements of fluorescence quantum yield using integral sphere are discussed from several aspects, such as parameters setting of instrument (step, integral time and slit width), and excitation wavelength. The tests showed that the accuracy of fluorescence quantum yield doesn’t have liner relations with the changes of step and integral time. Thus the test duration could be reduced by choosing larger step and shorter integral time. More obvious influence on the high fluorescence quantum yield compound was observed when changing the intensity of fluorescence, so the fluorescence intensity must be controlled not too high when testing the high fluorescence quantum yield compounds. The shifts of excitation wavelength shows a significant impact on the value of fluorescence quantum yield, so fixed the excitation wavelength was recommended in the fluorescence quantum yield test. This study provides some references for obtaining reliable quantum yield data.
Keywords: integrating sphere;fluorescence quantum yield;optimized test conditions
2021, 47(10):59-62,74 收稿日期: 2020-11-17;收到修改稿日期: 2021-01-24
基金项目: 聚合物分子工程国家重点实验室仪器类开放课题(KVH1717329/001/002)
作者简介: 胡晓月(1984-),女,辽宁沈阳市人,实验师,博士,主要从事仪器的分析测试与管理工作
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