作者：李响丽, 张凤梅, 范多青, 蔡昊城, 高莉, 王庆华, 胡燕, 李超

作者单位：云南中烟工业有限责任公司技术中心，云南 昆明 650231

关键词：卷烟爆珠;壁材;合成着色剂;高效液相色谱

摘要：

该文开发一种高效液相色谱-二极管阵列检测器（HPLC-PDA）同时测定卷烟爆珠中10种合成着色剂（柠檬黄、苋菜红、胭脂红、日落黄、诱惑红、亮蓝、酸性红、赤藓红、靛蓝、喹啉黄）含量的方法。样品经热水提取后通过高效液相色谱分析，以色谱峰保留时间和紫外可见光谱进行定性。流动相采用乙腈-甲醇-乙酸铵（0.02 mol/L）水溶液，检测中使用梯度洗脱，检测波长分别为427 nm、511 nm、625 nm和529 nm，外标法定量。目标化合物在0.2~25 mg/L溶度范围内线性关系良好，相关系数均大于0.99，低中高3水平的加标回收率在82.1%~105.7%之间，相对标准偏差在1.0%~5.7%, 检出限为3.1~10.8 mg/kg。该方法具有简单、快速、准确的特点，能同时检测出烟用爆珠壁材中10种水溶性着色剂。该文能够为卷烟产品开发和安全评价提供技术支撑。

Simultaneous determination of 10 water-soluble synthetic colorants in cigarette bead wall materials by high performance liquid chromatography-diode array
LI Xiangli, ZHANG Fengmei, FAN Duoqing, CAI Haocheng, GAO Li, WANG Qinghua, HU Yan, LI Chao
R&D Center of China Tobacco Yunnan Industrial Co., Ltd., Kunming 650231, China
Abstract: In this study, a high performance liquid chromatography-photo-diode array (HPLC-PAD) method for simultaneous determination of 10 colorants (tartrazine, amaranth, ponceau, sunset yellow, allura red, brilliant blue, acid red 52, erythrosin, indigo, quinoline yellow) in breakable capsules of cigarette has been development. The samples were extracted by thermal water and analyzed by HPLC, then qualitative by retention time of chromatographic peak and UV–visible spectroscopy respectively. Acetonitrile/methanol/ammonium acetate aqueous solution (0.02 mol/L) was used as mobile phase. Then, gradient elution were used in detection, wavelength used in detection were 427 nm, 511 nm, 625 nm and 529 nm respectively. And quantitative analysis by external standard method. In the ranged of 0.2 to 25 mg/L, the target compounds had good linear relationship, and correlation coefficients were above 0.99. The standard addition recovery with low, middle and high levels were between 82.1% and 105.7%. And the relative standard deviations were between 1.0% and 5.7%. Then, the limits of detection were in the range of 3.1 to 10.8 mg/kg. This method has the characteristics of simple, rapid and accurate, and it could simultaneous determination of 10 colorants in breakable capsules of cigarette. Overall, the development of this method could provide technique support for cigarette products development and safety evaluation.
Keywords: breakable capsules of cigarette;wrapped materials;colorants;high performance liquid chromatography
2021, 47(3):76-81  收稿日期: 2020-04-23;收到修改稿日期: 2020-06-05
基金项目: 云南中烟重点科技项目（2018JC02）
作者简介: 李响丽(1978-),女,云南昆明市人,工程师,硕士,主要从事烟用材料分析研究工作
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