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首页>《中国测试》期刊>本期导读>采用荧光光谱法研究DDAF与BSA的相互作用

采用荧光光谱法研究DDAF与BSA的相互作用

127    2019-07-26

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作者:王秀文, 王颖莉, 于茜, 裴晓丽

作者单位:山西中医药大学, 山西 晋中 030619


关键词:双癸基二甲基甲酸铵;牛血清白蛋白;荧光光谱法;三维荧光光谱法


摘要:

研究新型反离子季铵盐DDAF与BSA的相互作用。通过单因素法,选出适宜的作用时间、Na+浓度和pH值;并采用荧光光谱法和三维荧光光谱法研究DDAF与BSA的相互作用机制。作用时间15 min、钠离子浓度为0.04 mol/L、pH值为待测液的初始pH值为适宜的实验条件;不同温度(290.15,296.15,303.15,310.15 K)下二者的双分子猝灭常数(Kq)分别为7.03×1010,8.53×1010,1.09×1011,1.12×1011 L/(mol·s);结合位点数(n)分别为1.17、0.84、0.97和1.02;$\Delta H$和$\Delta S$均大于0;三维荧光光谱显示,加入DDAF后,BSA的荧光峰发生蓝移,且荧光强度下降16.63%。DDAF和BSA为混合猝灭;二者以1:1结合;二者间的作用力是疏水作用力;DDAF使BSA氨基酸残基周围的疏水环境增强。


The study on the interaction between DDAF and BSA by fluorescence spectrometry
WANG Xiuwen, WANG Yingli, YU Qian, PEI Xiaoli
Shanxi University of Chinese Medicine, Jinzhong 030619, China
Abstract: The interaction mechanism between DDAF and bovine serum albumin(BSA) was studied. The suitable action time, sodiumion strength and pH were selected by single factor experiment. Under these suitable conditions, the fluorescence intensity of the BSA/DDAF system under 290.15 K, 296.15 K, 303.15 K and 310.15 K were measured by fluorescence spectrometry. The action time was 15 min, the sodium ion concentration was 0.04 mol/L, and the pH was the initial pH of the liquid to be measured as the appropriate experimental conditions. The two-molecule quenching constants (Kq) at different temperatures were calculated to be 7.03×1010 L/(mol·s), 8.53×1010 L/(mol·s), 1.09×1011 L/(mol·s), and 1.12×1011 L/(mol·s), respectively. Combined bit points (n) are 1.17, 0.84, 0.97 and 1.02, respectively. Both $\Delta H$ and $\Delta S$ are greater than 0. The three-dimensional fluorescence spectrum showed a blue shift in the fluorescence peak of BSA after the DDAF was added, and the fluorescence intensity decreased by 16.63%. The quenching mechanism of DDAF for BSA is mixed quenching. The combination ratio is 1:1. The force between the two is a typical hydrophobic force. DDAF enhances the hydrophobic environment around BSA amino acid residues.
Keywords: DDAF;BSA;fluorescence spectrometry;three-dimensional fluorescence
2019, 45(7):80-86  收稿日期: 2018-11-23;收到修改稿日期: 2019-01-15
基金项目: 山西省留学回国人员科技活动择优资助项目(2017066);太原科技大学博士启动基金(20162015);山西省"1331工程"工程(技术)研究中心建设计划(晋教科[2017]14号)
作者简介: 王秀文(1981-),女,山西太谷县人,讲师,硕士,主要从事中药及其制剂的质量研究
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