作者：张廷贵, 邓其馨, 林艳, 周培琛, 张鼎方, 刘江生, 刘泽春, 许寒春

作者单位：福建中烟工业有限责任公司技术中心, 福建 厦门 361022

关键词：液相色谱–串联质谱;烟草;肌醇;奎尼酸

摘要：

建立同时测定烟草中肌醇和奎尼酸的液相色谱–串联质谱（LC-MS/MS）方法。采用0.1 mol/L的乙酸铵水溶液对烟末进行超声提取后，经0.22 μm水相滤膜过滤后直接进行LC-MS/MS分析。结果表明：肌醇在0.11~25 mg/g和奎尼酸在0.18~25 mg/g范围内，线性相关系数均≥0.999 0。肌醇的回收率在95.9%~98.6%之间，相对标准偏差（RSD）小于1.9 %（n=6）；奎尼酸的回收率在95.2%~97.4%之间，相对标准偏差（RSD）小于2.1 %（n=6）。肌醇的方法检出限（LOD）为0.035 mg/g，定量限（LOQ）为0.11 mg/g；奎尼酸的方法检出限（LOD）为0.055 mg/g，定量限（LOQ）为0.18 mg/g。该方法准确、灵敏，适合用于烟草中肌醇和奎尼酸的测定。

Simultaneous determination of inositol and quinic acid in tobacco with liquid chromatography tandem mass spectrometry
ZHANG Tinggui, DENG Qixin, LIN Yan, ZHOU Peichen, ZHANG Dingfang, LIU Jiangsheng, LIU Zechun, XU Hanchun
Technology Center, China Tobacco Fujian Industrial Co., Ltd., Xiamen 361022, China
Abstract: The aim was to establish a high performance liquid chromatography trap tandem mass spectrometry (HPLC-MS/MS) method for simultaneous determination inositol and quinic acid in tobacco.The sample wasextracted by 0.1 mol/L ammonium acetate-water solution, and the extract was directlyanalyzed by LC-MS/MS after filtering with a 0.22 μm aqueous phase filtration membrane. The results showed that the linear correlation coefficientof inositolwas above 0.999 0 in the range of 0.11~25 mg/g, and the recovery of inositolwas from 95.9 % to 98.6 %, the relative standard deviations (RSDs) lower than 1.9%(n=6); the linear correlation coefficientofquinic acidwas above 0.999 0 in the range of 0.18~25 mg/g, the recovery of quinic acidwas from 95.2 % to 97.4 %, the relative standard deviations (RSDs) lower than 2.1%(n=6). The limit of detection (LOD) and limit of quantity (LOQ) of inositol were 0.035 mg/g and 0.11 mg/g, respectively; The LOD and LOQ of quinic acid were 0.055 mg/g and 0.18 mg/g, respectively. The method is verified with good efficiency, sensitivity and selectivity, and can be successfully applied insimultaneous determination of the inositol and quinic acid in varieties of tobacco samples.
Keywords: LC-MS/MS;tobacco;inositol;quinic acid
2019, 45(7):61-65  收稿日期: 2018-10-21;收到修改稿日期: 2018-11-26
基金项目: 中国科协青年人才托举工程CAST（2016QNRC001）
作者简介: 张廷贵(1985-),男,福建龙岩市人,工程师,主要从事烟草化学研究
参考文献
[1] SERBAN C M, MICHAEL F D. Formation of dihydroxybenzenes in cigarette smoke[J]. Contributions to Tobacco Research, 2012, 25(2):396-408
[2] 杨保刚, 麦成华, 张辉. 气相色谱法测定化妆品中肌醇的含量[J]. 香精香料化妆品, 2011(3):33-34
[3] 刘礼兵, 徐琪寿. 奎尼酸的生物合成与应用[J]. 生物技术通讯, 2005, 16(6):693-695
[4] SERBAN C M, MICHAEL F D. Analysis of quinicacid and of myo-Inositol in tobacco[J]. Contributions to Tobacco Research, 2012, 25(4):499-506
[5] 周敏, 陈亚波, 杨彤. 微生物法测定维生素功能饮料中的肌醇含量[J]. 安徽农业科学, 2014, 42(7):2123-2015
[6] ANAB B, ROSA MJ, ROSAM A. Simultaneous determination of five1, 4-dihydropyridine sinpharmaceutical for mulations by high-performanceliquid chromatography ampere metric detection[J]. J Chromatogr A, 2004, 1031(1-2):275-280
[7] 刘珊珊, 吴琼珠. 高效液相色谱法测定赖氨肌醇维B12口服溶液中维生素B12及肌醇的含量[J]. 药学与临床研究, 2007, 15(5):371-374
[8] 高树刚, 申书昌, 王文波, 等. 气相色谱法测定大豆磷脂中磷脂酰肌醇[J]. 理化检验-化学分册, 2007, 43(12):1020-1021
[9] 食品安全国家标准食品中肌醇的测定:GB 5009.270-2016[S]. 北京:中国质检出版社,2016.
[10] JIN G, SHI YF, XU CB, et al. Data on the optimization of a GC-MSprocedure for the determination of total plasmamyo-inositol[J]. Data Brief, 2016(8):1040-1043
[11] SUN S H, WANG H, XIE J P, et al. Simultaneous determination of rhamnose, xylitol, arabitol, fructose, glucose, inositol, sucrose, maltose in jujube(Zizyphus jujube Mill.) extract:comparison of HPLC-ELSD, LC-ESI-MS/MS and GC-MS[J]. Chem Centr J, 2016(10):25
[12] PERELLO J, ISERN B, COSTA-BAUZA A, et al. Determination of myo-inositol inbiological samples by liquid chromatography-mass spectrometry[J]. JChromatogr B Anal Technol Biomed Lif Sci, 2004, 802(2):367-370
[13] KIM J, HOPPEL CL. Comprehensive approach to the quantitative analysisof mitochondrial phospholipids by HPLC-MS[J]. J Chromatog B-AnalTechnol Biomed Lif Sci, 2013(912):105-114
[14] JINHO S, JUNGMIN P, HAJUNG K, et al. Development rapid analyticalmethds for inositol as a trace componentby HPLC and LC-MS/MS ininfant formula[J]. Korean J Food Sci, 2015, 35(4):466-472
[15] 房子舒, 黄传峰. 食品中肌醇的检测方法研究进展[J]. 食品安全质量检测学报, 2019, 10(1):14-18
[16] 刘泽春, 黄朝章, 邓其馨, 等. 烟用香精香料中奎尼酸的测定[J]. 香料香精化妆品, 2014, 10(4):29-32
[17] 王有志, 罗奇志, 罗佳. 高效液相色谱-电喷雾串联四极杆质谱法测定双黄连粉针剂中奎尼酸的含量[J]. 南方医科大学学报, 2010, 30(2):401-402