作者：程娟1,2, 潘天颖3, 吴郝娟2, 谢江1,2, 许文明3
作者单位：1. 西南医科大学临床医学院, 四川 泸州 646000;
2. 成都市第三人民医院, 四川 成都 610031;
3. 四川大学华西第二医院, 四川 成都 610041
关键词：脑源性神经营养因子;体外受精;胎盘厚度;ABC量表
摘要：
收集体外受精（in vitro fertilization， IVF）与自然受孕分娩儿童脐血和外周血，提取血清，采用酶联免疫吸附测定法（enzyme-linked immunosorbent assay， ELISA）检测血清脑源性神经营养因子（brain-derived neurotrophic factor， BDNF）水平，并进行自闭症儿童行为量表（autism behavior checklist， ABC）评分，彩色多普勒超声对妊娠11周及21周的胎盘厚度进行测量，了解体外受精及自然受孕儿童神经行为发育的差异。结果显示，体外受精分娩儿童血清BDNF水平低于自然受孕儿童（P<0.01），ABC量表评分高于自然受孕儿童（P<0.05），孕21周胎盘厚度增厚（P<0.05）。这提示体外受精可能会影响胎盘发育，并导致儿童神经行为的改变。
A study of neurobehavioral development in children conceived by in vitro fertilization
CHENG Juan1,2, PAN Tianying3, WU Haojuan2, XIE Jiang1,2, XU Wenming3
1. Clinical Medical College, Southwest Medical University, Luzhou 646000, China;
2. The Third People's Hospital of Chengdu, Chengdu 610031, China;
3. The Second Affiliated West China Hospital of Sichuan University, Chengdu 610041, China
Abstract: The umbilical cord and peripheral blood of naturally conceived and in vitro fertilization (IVF) children were collected, then serum was extracted. All children were detected the serum brain-derived neurotrophic factor (BDNF) levels with the enzyme-linked immunosorbent assay (ELISA).The neurobehaviour scores were evaluated with the autism behavior checklist (ABC). Color Doppler Ultrasonography was used to measure the placenta thickness at 11 and 21 weeks of pregnancy. To understand the difference of neurobehavioral development between in vitro fertilization and natural conception. The results showed that serum BDNF level of in vitro fertilization children was lower than that of natural conception children (P<0.01), ABC scale score was higher than that of natural conception children (P<0.05), and placental thickness increased at 21 weeks of gestation (P<0.05). This suggests that in vitro fertilization may affect placental development and lead to changes in neural behavior in children.
Keywords: brain-derived neurotrophic factor;in vitro fertilization;placental thickness;autism behavior checklist
2019, 45(3):65-68,74 收稿日期: 2018-11-05;收到修改稿日期: 2018-12-18
基金项目: 国家自然科学基金项目（81771642）；四川省科技厅科研项目（2016JY0185）
作者简介: 程娟(1992-),女,四川宜宾市人,硕士研究生,专业方向为行为发育异常
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