作者：耿琦¹, 余洋¹, 刘绒梅¹, 田圆圆¹, 李玺洋¹, 梁鹏宽¹, 石海春², 柯永培², 孙群¹

作者单位：1. 四川大学生命科学学院 生物资源与生态环境教育部重点实验室, 四川 成都 610064;
2. 四川农业大学农学院, 四川 成都 611130

关键词：淡紫灰链霉菌S3-5;田间试验;稳定剂;紫外保护剂

摘要：

尾孢菌（Cercospora zeae-maydis）是引起玉米灰斑病的病原真菌，淡紫灰链霉菌（Streptomyces lavendulae ）S3-5为课题组前期研究筛选出的尾孢菌拮抗菌株。为探究S3-5在田间对玉米灰斑病的防治效果，并研制有效防控玉米灰斑病的生防制剂，该研究进行田间试验，并对S3-5稳定剂及紫外保护剂进行筛选。结果表明，初步应用S3-5孢子悬液于田间栽培品种正红311上，发现其对玉米灰斑病具有一定的防治效果，喷施两次的效果更显著（P0.05）；最佳稳定剂为2.5%甲基纤维素，最佳紫外保护剂为0.3%异维C钠。可为S3-5制剂的后续研究奠定基础。

Effect exploring of anti-gray leaf spot on maize in the field and screening of adjuvants for the biocontrol agent of Streptomyces lavendulae S3-5

GENG Qi¹, YU Yang¹, LIU Rongmei¹, TIAN Yuanyuan¹, LI Xiyang¹, LIANG Pengkuan¹, SHI Haichun², KE Yongpei², SUN Qun¹

1. Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China;
2. Agronomy College, Sichuan Agricultural University, Chengdu 611130, China

Abstract: Cercospora zeae-maydis is the cause agent of gray leaf spot disease (GLS) in maize, and Streptomyces lavendulae S3-5 screened by the laboratory previously shows effective inhibition against the growth of C. zeae-maydis. The aim of this study was to assess the ability of S3-5 to control GLS and to develop a formulation of biocontrol agent with S3-5 to suppress GLS. The experiments were conducted on the application of S3-5 spore suspension in the field, as well as screening stabilizers and UV protectants. As a result, the significant control effect was obtained by applying the S3-5 spore suspension twice on maize cultivar Zhenghong 311, 2.5% methylcellulose and 0.3% D-Sodium isoascorbiate were found as the optimum stabilizer and UV protectant, respectively. This study established foundations for the development of S3-5 biocontrol agent in the future.

Keywords: Streptomyces lavendulae S3-5;field trial;stabilizers;UV protectants

2018, 44(6): 54-59  收稿日期: 2018-03-02;收到修改稿日期: 2018-04-10

基金项目: 国家国际合作项目（2015DFR31060）

作者简介: 耿琦(1993-),男,陕西镇安县人,硕士研究生,专业方向为应用微生物。

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