作者：李波¹, 崔杰华¹, 王颜红¹, 王俊伟², 王伟³

作者单位：1. 中国科学院沈阳应用生态研究所, 辽宁沈阳 110016;
2. 沈阳农业大学, 辽宁沈阳 110161;
3. 沈阳药科大学, 辽宁沈阳 110016

关键词：食品; 微波消解; 原子荧光光谱法; 原子吸收光谱法; 金属元素

摘要：

针对食品检测前处理时间和检测周期过长的难题,用硝酸及双氧水作为消解溶剂,在设定的微波消解条件下,可以将样品消解完全,消解液中加入硫脲和L盐酸,不需任何处理可直接进行砷等7种元素的测定。硫脲将高价砷和汞离子还原为三价砷和汞原子,而对其它元素无影响,从而实现氢化物发生原子荧光法同时测定食品中砷、汞的含量,原子吸收火焰法测定食品中铜、锌、铁、镁、锰的含量。通过测定标准物质和加标回收实验,对方法进行验证,结果表明,方法简便快速、准确度与精密度令人满意。

Direct determinations of seven elements in food with one microwave digestion solution

LI Bo¹, CUI Jie-hua¹, WANG Yan-hong¹, WANG Jun-wei², WANG Wei³

1. Institute of Applied Ecology, Chinese Academy of Sciences, Shengyang 110016, China;
2. Shenyang Agricultural University, Shengyang 110161, China;
3. Shenyang Pharmaceutical University, Shengyang 110016, China

Abstract: According to the problem of the long processing time and testing cycle for food measurement, the feasibility of using one microwave digestion solution to investigate seven elements in food was discussed. with the microwave digesting procedure of this study, the samples were digested completely with nitric acid and hydrogen peroxide. After thiourea and hydrochloric acid were added to the digestion solution, seven elements can be determined without any other pretreatments.Thiourea could reduce highly charged As and Hg ions into As (Ⅲ) and Hg atoms respectively, and it didn't affect other element determinations. Thus, the contents of As and Hg in food could be determined with hydride generation-atomic fluorescence spectrometry (HG-AFS) simultaneously, and those of Cu、Zn、Fe、Mg、Plus Mn could be determined with atomic absorption spectrometry (AAS). The method accuracies were confirmed by standard samples analysis and recovery experiments. The results indicate this method is simple, rapid, accuracy and repeatable,and it is suitable for the content determinations of these seven elements in food.

Keywords: Food; Microwave digestion; HG-AFS; AAS; Metallic element

2008, 34(2): 89-91  收稿日期: 2007-3-8;收到修改稿日期: 2007-5-26

基金项目: 沈阳市科技计划项目(1063208-3-00)

作者简介: 李波(1976-),男,江苏徐州市人,工程师,主要从事食品安全、环境安全及分析检测技术工作。

参考文献

[1] GB/T 5009.11-2003, 食品中总砷和无机砷的测定[S].
[2] GB/T 5009.17-2003, 食品中总汞及有机汞的测定[S].
[3] GB/T 5009.13-2003, 食品中铜的测定[S].
[4] GB/T 5009.14-2003, 食品中锌的测定[S].
[5] GB/T5009.90-2003, 食品中铁、镁、锰的测定[S].
[6] 谢锋, 何锦林, 谭红, 等.微波高压硝酸硝化原子荧光法测定微量砷[J].贵州科学, 2005, 23(1):17-19.
[7] 刘春华.微波消解-原子荧光光谱法同时测定中成药中的砷和汞[J].现代科学仪器, 2004(1):37-39.
[8] 张军国. 利用微波消解和原子荧光光度法同时测定水样中的砷、汞[J].干旱环境监测, 2002, 16(4):201-202.
[9] 牟仁祥, 陈铭学, 应兴华, 等.微波消解-HG-AFS测定大米中痕量砷[J].光谱实验室, 2003, 20(6):852-855.
[10] 高燚, 杨海霞, 于卫荣.氢化物发生原子荧光光谱法同时测定海洋沉积物中的砷和汞[J]. 海洋水产研究, 2005, 26(4):53-59.
[11] 王爱平.一次微波消解原子荧光法测定土壤中砷汞[J].微量元素与健康研究, 2005, 22(6):44-45.
[12] 陈明岩, 徐立明, 骊书民, 等.微波消解-氢化物发生原子荧光法测定塑料原料及其制品中的砷、汞[J].化学分析计量, 2005, 14(4):19-21.
[13] 汪禄祥, 刘家富, 董宝生, 等.微波消解氢化物-原子荧光法同时测定土壤中的砷和汞[J].西南农业学报, 2006, 19(2):211-213.
[14] 张锂, 韩国才.氢化物发生原子荧光法测定环境水样品中的总汞砷铅[J].中国卫生检验杂志, 2006, 16(2):179-181.